"Life isn't about finding yourself. Life is about creating yourself."

George Bernard Shaw

Professional experiences


11.2022–present

Head of Laboratory at SGS S.A.

    –  SGS S.A.


07.2017–10.2022

Head of R&D and Lab Services at Xenometrix AG

    –  Xenometrix AG


09.2013–06.2017

SNF Postdoctoral fellow at Universität Zürich


01.2013–08.2013

Postdoc at Università Vita-Salute S. Raffaele


01.2009–12.2012

PhD student at Università Vita–Salute S. Raffaele       PhD student at the Open University


03.2008–12.2008

Scientist at Università degli Studi di Milano – Bicocca


11.2006–02.2008

Junior Scientist at Università degli Studi di Milano


Education


2009–2012:  PhD in Cellular and Molecular Biology

Università Vita–Salute San Raffaele (Milan, Italy)
in association with the Open University (London, England)

2003–2006:  Collegio di Milano

2004–2006:  Master's Degree in Biology

110/110 cum laude  (29.4/30.0 GPA)
Università degli Studi di Milano – Bicocca (Milan, Italy)

2001–2004:  Bachelor's Degree in Biology

110/110 cum laude  (29.9/30.0 GPA)
Università degli Studi di Milano – Bicocca (Milan, Italy)

2001–2004:  High school degree (Maturità Scientifica)

100/100 cum laude
Liceo Scientifico Statale Albert Einstein (Milan, Italy)
Download
PhD_DSpiliotopoulos.pdf
Adobe Acrobat Document 7.1 MB
Download
USR_PhD_DSpiliotopoulos.pdf
Adobe Acrobat Document 118.5 KB
Download
OU_PhD_DSpiliotopoulos.pdf
Adobe Acrobat Document 299.0 KB

Skills


Lab Management

Scientific Writing

Molecular Biology

Biochemistry

Biophysics

Microbiology

Cell Biology

Computational Chemistry

Programming

DNA and RNA extraction, gene cloning, site–directed mutagenesis, PCR, bacterial transformation, transfection

protein purification, chromatography, Western Blot, pull–down assays, enzyme assays

protein–ligand binding assays (ITC, DSF, TR–FRET, AlphaScreen assay), protein NMR spectroscopy

Ames MPF

stem and cancer cell culture, cell proliferation assays, immunofluorescence, flow cytometry, XenoScreen assay

molecular docking, Molecular Dynamics

Bash, Python


Certificates


edX courses:

   -  Data Science Orientation  (Microsoft)

   -  Data Science: R Basic  (HarvardX)

   -  Data Science: Visualization  (HarvardX)

   -  Data Science: Probability  (HarvardX)

Lab safety courses:

   -  Biosafety Officer (BSO) of Safety Level 1  (FOEN, b-safe Ltd.)

   -  Autoclaves and Waste Disposal  (FOEN, b-safe Ltd.)

ISO training:

   -  ISO 9001 2015  (Xenometrix AG)

 


Languages


English

German

Italian

Greek

excellent

intermediate

native speaker

native speaker

FCE (Feb 2000)


Some of the achievements I am proud of in my past jobs


at

Xenometrix

lab management (microbiology and cell biology laboratories)

• project management for interdisciplinary collaborative efforts

• development of assays

• drafting of a scientific manuscript (1 publication) and documents

• proactive mentoring and coordination of 2 lab technicians
invited speaker at international conferences and meetings


in the Caflisch
group

lab management (biochemistry and cell biology laboratories)

set up of a cell biology laboratory
• project management for interdisciplinary collaborative efforts

• proactive recruitment, mentoring and coordination of 5 trainees and 5 undergraduate students
• drafting of scientific manuscripts (13 publications) and grant proposals (raising >370,000 CHF)
invited speaker at international conferences
lectures to biology, chemistry, biochemistry and medical students
• identification and characterization of structure–based protein–protein interaction and enzyme inhibitors


in the Musco
group

 

• project management for interdisciplinary efforts

establishment of biochemical and biophysical assays
development of a user–friendly suite of scripts streamlining free energy calculations (GMXPBSA)
development of a user–friendly R package for the statistical analysis  (muma)
• drafting of scientific manuscripts (8 publications) and grant proposals (raising >54,000 EUR)
organization of international PhD meetings

in silico and in vitro investigation of protein–protein and protein–peptide interactions


in the Cattaneo group

• drafting of scientific manuscripts (2 publications) and grant proposals (raising >10,000 EUR)

lectures to biology and biotechnology students
• improvement of the protocol converting embryonic stem–derived murine Neural Stem cells into mature neurons
• establishment and implementation of genome editing by means of transposons and clonal isolation